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random hexanucleotide primer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher random hexanucleotide primer
    Random Hexanucleotide Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hexanucleotide+random+primers/random+hexanucleotide+primer/pmc10928104__41419_2024_6576_MOESM3_ESM-90-12-18
    Average 90 stars, based on 1 article reviews
    random hexanucleotide primer - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: Therapeutic Effects of Vitamin D Analogs on Cardiac Hypertrophy in Spontaneously Hypertensive Rats
    Article Snippet: Statistically different miRNAs were selected using Partek genomic suite 6.4 with thresholds of fold change ≥1.5 and P < 0.05. .. First-strand cDNAs were synthesized from 2 μg of total RNAs in a 20 μl reaction using Moloney murine leukemia virus reverse transcriptase (Invitrogen) and hexanucleotide random primers. .. The first strand cDNA served as the template for PCR performed using a BioRad DNA Engine or an Applied Biosystems 7900 Real Time PCR System with a SYBR green PCR reagent kit (Applied Biosystems, Foster City, CA) as described previously.

    Article Title: Therapeutic Effects of Vitamin D Analogs on Cardiac Hypertrophy in Spontaneously Hypertensive Rats
    Article Snippet: Statistically different miRNAs were selected using Partek genomic suite 6.4 with thresholds of fold change ≥1.5 and P < 0.05. .. RT-PCR First-strand cDNAs were synthesized from 2 μg of total RNAs in a 20 μl reaction using Moloney murine leukemia virus reverse transcriptase (Invitrogen) and hexanucleotide random primers. .. The first strand cDNA served as the template for PCR performed using a BioRad DNA Engine or an Applied Biosystems 7900 Real Time PCR System with a SYBR green PCR reagent kit (Applied Biosystems, Foster City, CA) as described previously.

    Article Title: Impaired Glomerular Maturation and Lack of VEGF165b in Denys-Drash Syndrome
    Article Snippet: .. cDNA was synthesized using Superscript II reverse transcriptase and hexanucleotide random primers (Invitrogen). ..

    Article Title: Targeted Expression of Human Vitamin D Receptor in Adipocytes Decreases Energy Expenditure and Induces Obesity in Mice
    Article Snippet: .. Quantitative Real-time RT-PCR—First-strand cDNAs were synthesized from 2 g of total RNAs in a 20- l reaction using Moloney murine reverse transcriptase (Invitrogen) and hexanucleotide random primers. .. The first-strand cDNAs served as the template for PCR amplification, whichwas carried out using the LightCycler 480 Real-time PCR System (Roche Applied Science).

    Virus:

    Article Title: Therapeutic Effects of Vitamin D Analogs on Cardiac Hypertrophy in Spontaneously Hypertensive Rats
    Article Snippet: Statistically different miRNAs were selected using Partek genomic suite 6.4 with thresholds of fold change ≥1.5 and P < 0.05. .. First-strand cDNAs were synthesized from 2 μg of total RNAs in a 20 μl reaction using Moloney murine leukemia virus reverse transcriptase (Invitrogen) and hexanucleotide random primers. .. The first strand cDNA served as the template for PCR performed using a BioRad DNA Engine or an Applied Biosystems 7900 Real Time PCR System with a SYBR green PCR reagent kit (Applied Biosystems, Foster City, CA) as described previously.

    Article Title: Therapeutic Effects of Vitamin D Analogs on Cardiac Hypertrophy in Spontaneously Hypertensive Rats
    Article Snippet: Statistically different miRNAs were selected using Partek genomic suite 6.4 with thresholds of fold change ≥1.5 and P < 0.05. .. RT-PCR First-strand cDNAs were synthesized from 2 μg of total RNAs in a 20 μl reaction using Moloney murine leukemia virus reverse transcriptase (Invitrogen) and hexanucleotide random primers. .. The first strand cDNA served as the template for PCR performed using a BioRad DNA Engine or an Applied Biosystems 7900 Real Time PCR System with a SYBR green PCR reagent kit (Applied Biosystems, Foster City, CA) as described previously.

    Reverse Transcription:

    Article Title: Therapeutic Effects of Vitamin D Analogs on Cardiac Hypertrophy in Spontaneously Hypertensive Rats
    Article Snippet: Statistically different miRNAs were selected using Partek genomic suite 6.4 with thresholds of fold change ≥1.5 and P < 0.05. .. First-strand cDNAs were synthesized from 2 μg of total RNAs in a 20 μl reaction using Moloney murine leukemia virus reverse transcriptase (Invitrogen) and hexanucleotide random primers. .. The first strand cDNA served as the template for PCR performed using a BioRad DNA Engine or an Applied Biosystems 7900 Real Time PCR System with a SYBR green PCR reagent kit (Applied Biosystems, Foster City, CA) as described previously.

    Article Title: Physiopathology of dental rickets in vitamin D receptor-ablated mice.
    Article Snippet: 1α25(OH)2vitaminD3 and its nuclear receptor, VDR, are essential for normal tooth development.. However, the relative contributions of the direct vs. indirect effects of vitamin D action on odontogenesis are unclear.. The aim of this study was to discriminate among the specific roles of 1α25(OH)2 vitaminD3, calcemia/phosphatemia, and the maternal environment in mouse VDR null mutants.

    Article Title: Murine Cytomegalovirus Virion-Associated Protein M45 Mediates Rapid NF-κB Activation after Infection
    Article Snippet: .. Following cDNA synthesis using SuperScript III reverse transcriptase (Invitrogen), hexanucleotide random primers (Invitrogen), and the RNase inhibitor RNasin (Promega), reverse transcription-quantitative PCR (qRT-PCR) was performed using TaqMan PCR and the Universal Probe Library (Roche) or SYBR green PCR. ..

    Article Title: Therapeutic Effects of Vitamin D Analogs on Cardiac Hypertrophy in Spontaneously Hypertensive Rats
    Article Snippet: Statistically different miRNAs were selected using Partek genomic suite 6.4 with thresholds of fold change ≥1.5 and P < 0.05. .. RT-PCR First-strand cDNAs were synthesized from 2 μg of total RNAs in a 20 μl reaction using Moloney murine leukemia virus reverse transcriptase (Invitrogen) and hexanucleotide random primers. .. The first strand cDNA served as the template for PCR performed using a BioRad DNA Engine or an Applied Biosystems 7900 Real Time PCR System with a SYBR green PCR reagent kit (Applied Biosystems, Foster City, CA) as described previously.

    Article Title: Impaired Glomerular Maturation and Lack of VEGF165b in Denys-Drash Syndrome
    Article Snippet: .. cDNA was synthesized using Superscript II reverse transcriptase and hexanucleotide random primers (Invitrogen). ..

    Article Title: Targeted Expression of Human Vitamin D Receptor in Adipocytes Decreases Energy Expenditure and Induces Obesity in Mice
    Article Snippet: .. Quantitative Real-time RT-PCR—First-strand cDNAs were synthesized from 2 g of total RNAs in a 20- l reaction using Moloney murine reverse transcriptase (Invitrogen) and hexanucleotide random primers. .. The first-strand cDNAs served as the template for PCR amplification, whichwas carried out using the LightCycler 480 Real-time PCR System (Roche Applied Science).

    Article Title: Role of RANKL (TNFSF11)-Dependent Osteopetrosis in the Dental Phenotype of Msx2 Null Mutant Mice
    Article Snippet: Tissues were directly immersed in RNA extraction solution (Tri-Reagent; Euromedex, Souffelweyersheim, France), and the extraction was performed according to the manufacturer's instructions. .. For classical RT-PCR, reverse transcription was performed on 1 μg of total RNA with Superscript II (Gibco, Cergy-Pontoise, France) and hexanucleotide random primers (Gibco), and PCRs were done with Eurobiotaq (Eurobio, Courtaboeuf, France), following the manufacturer's instructions. ..

    cDNA Synthesis:

    Article Title: Murine Cytomegalovirus Virion-Associated Protein M45 Mediates Rapid NF-κB Activation after Infection
    Article Snippet: .. Following cDNA synthesis using SuperScript III reverse transcriptase (Invitrogen), hexanucleotide random primers (Invitrogen), and the RNase inhibitor RNasin (Promega), reverse transcription-quantitative PCR (qRT-PCR) was performed using TaqMan PCR and the Universal Probe Library (Roche) or SYBR green PCR. ..

    Polymerase Chain Reaction:

    Article Title: Murine Cytomegalovirus Virion-Associated Protein M45 Mediates Rapid NF-κB Activation after Infection
    Article Snippet: .. Following cDNA synthesis using SuperScript III reverse transcriptase (Invitrogen), hexanucleotide random primers (Invitrogen), and the RNase inhibitor RNasin (Promega), reverse transcription-quantitative PCR (qRT-PCR) was performed using TaqMan PCR and the Universal Probe Library (Roche) or SYBR green PCR. ..

    SYBR Green Assay:

    Article Title: Murine Cytomegalovirus Virion-Associated Protein M45 Mediates Rapid NF-κB Activation after Infection
    Article Snippet: .. Following cDNA synthesis using SuperScript III reverse transcriptase (Invitrogen), hexanucleotide random primers (Invitrogen), and the RNase inhibitor RNasin (Promega), reverse transcription-quantitative PCR (qRT-PCR) was performed using TaqMan PCR and the Universal Probe Library (Roche) or SYBR green PCR. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Therapeutic Effects of Vitamin D Analogs on Cardiac Hypertrophy in Spontaneously Hypertensive Rats
    Article Snippet: Statistically different miRNAs were selected using Partek genomic suite 6.4 with thresholds of fold change ≥1.5 and P < 0.05. .. RT-PCR First-strand cDNAs were synthesized from 2 μg of total RNAs in a 20 μl reaction using Moloney murine leukemia virus reverse transcriptase (Invitrogen) and hexanucleotide random primers. .. The first strand cDNA served as the template for PCR performed using a BioRad DNA Engine or an Applied Biosystems 7900 Real Time PCR System with a SYBR green PCR reagent kit (Applied Biosystems, Foster City, CA) as described previously.



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